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KMID : 0191120200350070084
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Journal of Korean Medical Science
2020 Volume.35 No. 7 p.84 ~ p.84
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Virus Isolation from the First Patient with SARS-CoV-2 in Korea
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Park Wan-Beom
Kwon Nak-Jung
Choi Su-Jin
Kang Chang-Kyung
Choe Pyoeng-Gyun
Kim Jin-Yong
Yun Ji-Young
Lee Gir-Won
Seong Moon-Woo
Kim Nam-Joong
Seo Jeong-Sun
Oh Myoung-Don
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Abstract
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Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.
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KEYWORD
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2019 Novel Coronavirus, Culture, Microscopy, Electron, Phylogeny, Korea
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